According to the coding sequence of GhTCP15 gene,a pair of primers were designed. The homologous sequence of GbTCP15 gene,was cloned from cotton(Gossypium barbadense L.)cultivar Xinhai 21 using reverse transcription PCR methods in this study. GbTCP15 gene contained open reading frame(ORF) of 1 056 bp in length, 351 amino acids residues with a predicted molecular mass of about 38 057.4 kD and a basic isoelectric point of 9.01,with a highly conservative TCP domain in the encoded putative protein. Amino acid sequence alignment revealed of GbTCP15 protein shared high degree of identity with other higher plant TCP proteins and the gene in evolution has proved to be conservative. Subcellular localization showed that GbTCP15 expressed possibly in cell nucleus. Thus, we speculated that GbTCP15 play a role on signal transduction and transcriptional regulatory during replication and transcription. The phylogenetic tree showed that GbTCP15 was located at the same distribute with Gossypium raimondii GrTCP15. The experment results of real time PCR exhibited that GbTCP15 had a higher expression level in stems and fibers (15 d). Above results suggested that GbTCP15 transcription factor may be involved in the cotton fiber and table fur development.