Based on the data of 560 quadrates, we studied the interspecific associations of 18 main plants with its frequency greater than or equal to 5% and experienced many times of waterlevel fluctuation in the waterlevel fluctuation zone of canyon landform area of the Three Gorges Reservoir through quantitative analysis method such as variance ratio test, Chisquare test, Jaccard index, Spearman rank correlation coefficient and so on. The ecological adaptability and the intrinsic association of different plants, and the stage of community succession were discussed to provide a scientific basis for vegetation restoration and reconstruction. (1) The significant positive association was found among overall main 18 plants because of similarities of ecological habit, ecological requirement and ecological niche overlap. Among 153 species pairs, 78 species pairs showed positive association and 75 species pairs showed negative association in the Chisquare test, while 74 species pairs showed positive correlation and 79 species pairs showed negative correlation in the Spearman rank correlation test. (2) A larger proportion of negative association in the Chisquare test and the Spearman rank correlation test indicated the habitat was not stable and obvious resource competition existed among different plants. (3) The species pair with Jaccard index of less than 0.20 accounted for 82% of the total, which suggested that most species pairs were not closely related and interspecific association was susceptible to the interference of external factors. In the actual habitat conditions, plant community was still in the stage of instability.