In Arabidopsis thaliana, the arsenic induced gene At4g13180 encoding protein is one of the members of the SDR superfamily (ShortChain Dehydrogenase/Reductase). Its overexpression can enhance the resistance of plants to hydrogen peroxide. Here we studied the At4g13180 gene expression pattern and the encoded protein subcellular localization by semiquantitative RTPCR, with transgenic plants expressing ProAt4g13180:GUS, At4g13180EGFP and At4g13180OE. Our results show that the At4g13180 gene has a very strong expression in tissues such as root, leaf, sepal, and stamen filamentas. At4g13180EGFP is located in the nucleus and cytoplasm. Our study lays a basis of further research on the role of the arsenic induced gene At4g13180 in A. thaliana.