Taking ‘Xiahui 8’ ［Prunus persica (L.) Batsch.］ as test materials, this paper studied the effects of herbicides (glyphosate and paraquat) on vegetative growth，mineral element absorption，related enzyme activities，photosynthesis and gummosis in peach. The results showed that: (1) on the 3rd year after spraying herbicides (glyphosate and paraquat), the starch content in leaves and branches decreased and the soluble sugar content in leaves and branch decreased significantly after spraying glyphosate. (2) The N, P, K and Fe element contents in leaves raised while the Mn element contents in leaves was decreased significantly after spraying glyphosate. (3) On the 3rd year after spraying paraquat, the electrolyte leakage rate，catalase (CAT) and peroxidase (POD) activities in leaves raised. Glyphosate showed obvious influence on photosynthesis of ‘Xiahui 8’. The leaf photosynthetic rate (P_n) between 14:00 and 16:00 was lower than that of the control. (4) Investigations in the field showed that the gummosis incidence and disease index of ‘Xiahui 8’ increased after spraying glyphosate and paraquat. We should avoid using systemic herbicides in peach orchard.