The gDNA sequence and cDNA sequence of the ZmNAC99 gene were cloned from maize (Zea mays) inbred lines with differentially drought resistance. We carried on the preliminary bioinformatics analysis, and RTPCR and qRTPCR techniques were used to analyze the gene expression patterns under drought stress. The results indicated that: (1) the gDNA of ZmNAC99 were 1 892～1 908 bp and cDNA was 1 188 bp,encoding 395 amino acids, the Nterminal of ZmNAC99 has a typical NAM domain. Phylogenetic analysis showed that ZmNAC99 belonged to the OsNAC3 subfamily of the NAC family; (2) RTPCR and qRTPCR analysis indicated that ZmNAC99 could be induced by drought stress treatment; The ciselement analysis further revealed that the putative promoter of the ZmNAC99 gene contained 2 droughtresponsive ciselements MBS and 1 low temperature responsive element LTR. (3) The amino acid sequence alignment analysis of ZmNAC99 from different droughtresistant maize inbred lines suggested that there were 4 aminoacid mutation, and we speculated that the difference of the structure may have an effect on the drought resistance of maize. These results suggest that ZmNAC99 may play an important role in the plants resistance to stress.