Species of genus Kobresia are the dominants and edificators in southeast Tibet alpine meadow, and play important roles in the livestock production and stabiligy of ecosystem. To study the adaptation of different characteristics of Kobresia plant to elevational gradient, we set eight sample belts along an elevational gradient, starting from forest line to the edge of alpine meadows, as increasing of elevation for every about 100 m at Dongda Mountains in Zuogong County. Species composition and coverage in every sample belts were investigated, and important values of three Kobresia species were calculated in the community based on relative coverage and frequency. At the same time, the epidermal cell shapes of adaxial and abaxial surface were observed, the stomatal length and guard cell width were measured and stomatal density was calculated, so as to observe the adaptability of Kobresia species to the elevation gradient. The results showed: (1) Common features of epidermis were investigated in three species using light microscopy: epidermis was wavy, and stomata apparatus was only distributed in the epidermis of abaxial surface. (2) The stomatal density of all three species presented to be a unimodal pattern along an elevational gradient, and obtained a maximum at the sample belt altitude of 4 537 m. Stomatal density of K. capillifolia (476.4 grasses per mm²) was lower than which of K. humilis (777.6 grasses per mm²), and higher than which of K. macrantha (414.3 grasses per mm²). (3) With the elevational gain, the stomata length of K. humilis(P<0.05)and K. capillifolia significantly increased, and guard cell width of K. humilis and K. capillifolia significantly decreased. By contrast, the stomata length of K. macrantha decreased with the gain of elevation, and guard cell width of which remained basically unchanged (P>0.05). (4)The stomatal density, length and guard cell width of K. humilis and K. capillifolia were significantly correlated with the elevational gradient, and the stomatal characteristics were highly sensitive to the change of elevational gradient, which was consistent with the high importance value in the community. Only stomatal density and length of K. macrantha were significantly correlated with the elevational gradient, and the stomatal characteristics were not highly sensitive to the change of elevational gradient, which was consistent with the low importance value in the community. The correlation between stomatal density, length and guard cell width of Kobresia species and elevational gradient showed the degree of adaptation to habitat at the elevational gradient. It can be seen that the stomatal characteristics of the three Kobresia species have different adaptability to the habitat along the elevational gradient, thus affecting their distribution range and species coverage in the community. K. humilis and K. capillifolia were sensitive to habitat changes, while K. macrantha was insensitive to habitat changes; the guard cell width, like the stomatal length, played an important role in adapting to the habitat.