The ‘New Sea 21’ of Gossypium barbadense was used as the experimental material. A pair of primers were designed according to the GhTCP14 gene sequence of upland cotton. A nucleotide sequence was obtained by RTPCR. The open reading frame was 1 221 bp, encoding 406 amino acids, molecular formula C₁₈₉₂H₂₉₅₀N₅₇₂O₆₂₀S₁₆, the predicted molecular weight was 44.134 6 kD, the isoelectric point was 6.88, and the amino acid sequence contained one highly conserved TCP domain and four low abundance complex regions. The comparison of amino acid sequence of GbTCP14 protein with other species TCP14 showed that the GbTCP14 protein of G. barbadense had a highly conserved TCP domain with the TCP14 protein in other plants, and the consistency between the sequences is high. The results of phylogenetic tree showed that the GbTCP14 gene of G. barbadense was distributed on the same branch as Grafted cotton GaTCP14 gene. Realtime quantitative PCR showed that the expression level of GbTCP14 gene was the highest at the 15th day after flowering, and the relative expression level was higher on the 5th day to the 20th day than that in other days. The expression level in petals and calyx was higher among different tissues, and the expression level in roots and stems was the lowest. GbTCP14 protein did not have transcriptional activity by yeast system transcriptional activation assays. The results showed that this study successfully cloned a TCP transcription factor from the island of cotton. The preliminary analysis of the gene has a good potential capacity, the specific function to be further studied.