54 EST sequences, belonging to Tau, Zeta, Theta and Phi classes, were mined from the transcriptome databases of Liriodendron. 4 EST sequences were selected for designing primers and corresponding genes, named LtGSTparCb, LtGSTZlike, LtGSTF13a, LcGSTT1, were cloned by reverse transcription PCR and rapid amplification of cDNA ends technology. The full cDNA length of LtGSTparCb, LtGSTZlike, LtGSTF13a and LcGSTT1 were 1 150, 932, 1 022 and 1 067 bp respectively. The prediction results of protein structure showed that the four proteins molecular weights were about 25.17, 25.34, 24.49 and 28.09 kD, respectively; the theoretical isoelectric points (pI) were 5.60, 6.53, 6.66, 9.20, which mean that LtGSTparCb, LtGSTZlike, LtGSTF13a were acidic protein and LcGSTT1 was basic protein; the instability indexes were 33.01, 36.97, 43.19, 47.96, so that LtGSTparCb and LtGSTZlike were classified as stable proteins, whereas, LtGSTF13a and LcGSTT1 proteins were unstable; Alpha helix was the mainly secondary structure component of the four proteins. The four proteins were predicted located at cytoplasm in Liriodendron. Expression profile by realtime PCR showed that they were expressed in all eight tissues studied, while the expression levels varied significantly among eight tissues. The expression of LtGSTparCb and LtGSTF13a in leaves was the highest while lower in other tissues. LtGSTZlike had the highest expression level in floral organs and lowest expression in leaves. LcGSTT1 had the highest expression level in leaves and lower in floral organs, which was least in stem and leaf buds. The results could lay a foundation for the further studies of function about GST family genes in Liriodendron.