Dehydration time and thawing temperature were explored to test the possibility of encapsulationvitrification in the cryopreservation of bryophytes collected from moist habitats. The results showed that the regeneration rate of S. ovatum can reach 42.41% when the materials were precultured at 4 ℃ for 3 days, loaded with 60% PVS₂ for 30 minutes and dehydrated with PVS₂ for 60 minutes at 0 ℃, then stored in liquid nitrogen for 24 hours and thawed at 40 ℃ for 2 minutes. There are no significant differences of morphological indicators between the pants before and after cryopreserved. The results demonstrated the feasibility of cryopreservation of bryophytes in humid environment by encapsulationvitrification.