Abstract:In this study, the new species Magnolia wufengensis ‘Jiaohong No.1’ was selected as the research material in Wufeng, Hubei. A SUPERMAN like gene MwZFP10 (GenBank accession MH037314) was cloned with 3′RACE method, based on the 645 bp core fragment related with floral development and the fragment was filtered out from differentially expressed genes based on preLaboratory cDNA library of Magnolia wufengensis. Bioinformatics analysis of MwZFP10 gene showed that the full length of MwZFP10 cDNA was 1 117 bp with an open reading frame (ORF) of 726 bp encoding 241 amino acids. The protein molecular formula is C1128H1760N322O383S14, molecular weight is 26.4 kD and isoelectric point is 4.42. The protein contains a conserved domain: the C2H2type zinc finger. Comparative analysis found that MwZFP10 belongs to the SUPERMANlike zinc finger protein, with the “QALGGH” in the N terminal and “L(I)DLXLR(K)L” in the C terminal. αhelix (Hh), extended chain (Ee) and βturn (Bt) constitute are the basic structure of the protein. The tertiary structure of the protein has a typical zinc finger spatial configuration. The protein is hydrophilic and mainly localized in the nucleus. The semiquantitative PCR and quantitative realtime PCR results showed that MwZFP10 was expressed in floral organs, leaf buds and bracts, and the pistil expression was higher in floral organs. MwZFP10 transgenic Arabidopsis thaliana is characterized by short and sterile plants, curling leaves, malformation of flower organs, etc. The gene MwZFP10 play an important role in the floral growth and development.