In this study, ‘Longjingchangye’ tea plant was used as tested material, and the cDNA sequence of the CsLEA5 gene in tea plant was cloned. The fulllength cDNA was 515 bp, including a 375 bp open reading frame that encodes 124 amino acids with a molecular weight of 13.5 kD and a theoretical pI of 5.92. Protein sequence analysis showed that CsLEA5 is a highly hydrophilic and stable protein, and contains a typical LEA_3 conserved domain, belonging to LEA_3 subfamily members of LEA protein. In addition, the promoter of CsLEA5 gene contains a variety of cisacting elements related to stress response, such as ethylene responsive element (ERE), stress responsive element (STRE), wound responsive element (wunmotif) and MYB, MYC transcription factor recognition sites, etc. The qRTPCR analysis showed that the expression of CsLEA5 gene had obvious tissue specificity, with the highest expression in leaves, followed by tender stem, and lower expression in other tissues and organs. Furthermore, the expression of CsLEA5 gene was induced by cold and drought stress, suggesting that it plays an important role in the response of tea plants to cold and drought stress. This study provides an important theoretical basis for understanding the resistance molecular mechanism of tea tree and screening the resistance candidate gene resources.