In this experiment, the effects of different concentrations of cadmium (0, 5 and 10 mg·L^-1) on the growth of 10 kinds of rootstock seedlings squash, the content of malondialdehyde (MDA), the content of Cd in shoots and the transfer coefficient (TF) were studied by means of nutrient solution culture. We used membership function method to comprehensively evaluate its ability and absorption and accumulation ability of Cd in order to screen excellent rootstocks The results showed that: (1) the whole plant biomass of ‘Duomei 109’, ‘Xinli 3’, ‘Qiangli No. 1’, ‘Heizi Nangua’ and ‘Yunnanheizi Nangua’ were significantly lower than that of control under 5 mg·L^-1 and 10 mg·L^-1 Cd treatments, and the biggest decreased were ‘Heizi Nangua’ and ‘Yunnanheizi Nangua’, which were the worst resistance. However, the biomass of ‘Yemu Yixiong’, ‘Qingjia Zhenjia’, ‘Jinyou 108’ and ‘Kangxian No. 1’ had no difference with the control, and the tolerance was the strongest. (2) Under the two concentrations of Cd treatments, the antioxidase activities in seedlings of ‘Jinyou 108’ was higher, and the MDA contents in roots and leaves were not significantly different from the control, indicating that it was not subjected to membrane lipid peroxidation injury. The antioxidase activity in ‘Yemu Yixiong’ seedlings was higher, and the increase of MDA content was smaller, which was 1.01~1.56 times of the control, indicating that the membrane lipid peroxidation injury was relatively small. The antioxidase activity of ‘Domei 109’, ‘Qiangli 1’ and ‘Yunnan Yunnanheizi pumpkin’ seedlings were decreased significantly under the 5 mg·L^-1 and 10 mg·L^-1 Cd treatments, and the MDA content increased greatly, which was 1.30-2.46, 1.33-3.11, 1.45-3.24 times higher than that of the control, respectively, and the most severely affected. (3) The Cd content and TF of ‘Jinyou 108’ and ‘Qingjia Zhenjia’ were lower, and the Cd absorption capacity was the weakest under Cd treatment, followed by ‘Yemu Yixiong’. However, ‘Lianglijia’, ‘Heizi Nangua’ and ‘Yunnanheizi Nangua’ were the highest, and the Cd absorption capacity was the strongest. (4) The comprehensive evaluation results of the membership function values showed that the tolerance of 10 variety pumpkin seedlings for rootstocks to Cd was as follows: ‘Jinyou 108’ >‘Yemu Yixiong’ >‘Xinli 3’ >‘Qingjia Zhenjia’> ‘Kangxian No. 1’> ‘Duomei 109’ >‘Heizi Nangua’ > ‘Lianglijia’> ‘Qiangli No. 1’ > ‘Yunnanheizi Nangua’. The studies showed that the tolerance of rootstock pumpkin varieties to cadmium stress was significantly different, and ‘Jinyou 108’ was the best, which has good growth potential, strong tolerance, and the capacity of cadmium absorption and transport was the weakest under cadmium stress.