In this paper, 50 subtropical evergreen deciduous broadleaved mixed forest plots in the Xingdoushan National Nature Reserve of Hubei Province were studied. The leaf function of different life forms (shrubs, trees and lianas) and soil nutrients were determined by sample analysis. The determination was carried out, and the effects of soil nutrients on leaf functional traits of different lifetype woody plants were analyzed by redundancy analysis (RDA) and stepwise regression. The results show that: (1) the mean value of specific leaf area (SLA) of shrubs was the highest (319.87 cm·g^-1), and the average leaf dry matter content (LDMC) of trees was the highest (0.40 g·g^-1), leaf area (LA) of lianas, leaf thickness (LT), leaf total nitrogen content (LTN) and leaf total phosphorus content (LTP) mean value are the highest, They are: 105.07 cm², 0.24 mm, 20.10 mg·kg^-1, 1.19 mg·kg^-1; The LDMC and LTN distribution of the three life forms are concentrated, while the distribution of LA, SLA and LTN is right partial, but the distribution of LT and LTP are left partial, while that of shrubs and lianas are right partial distributed. (2) The pH value of soil in the study area is 3.81~4.95, all of which are acidic soil, and the content of soil organic matter is between 4.57% and 12.05%, with an average value of 7.79%; Soil rapidly available potassium and Soil availablenitrogen were as follows: 64.52~339.29 and 82.83~678.15 mg·kg^-1, Soil total N content, Soil total phosphorus content and Soil rapidly available phosphorus were as follows: 5.48 mg·kg^-1、0.46 mg·kg^-1 and 0.39 mg·kg^-1. (3) Among variation of leaf traits in three different life forms, soil nutrientshad the highest impacton liana variation, explaining 24% of them, next is shrubs (15%), and the lowestweretrees (13%). (4) Trees and liana are greatly affected by soil organic matter, and shrubs are greatly affected by rapidly available potassium. Although the main factors affecting functional traits of different life types are different, the main influencing factors are soil organic matter, soil total phosphorus, and rapidly available potassium. Research suggests: The soil of Xingdoushan National Nature Reserve can explain the variation of leaf functional traits of different life types of plants to some extent, and the influence degree is different. This difference is beneficial to species coexistence and biodiversity maintenance in this area. At the same time, the study on the relationship between leaf function and soil nutrients of different life type plants in subtropical region was made up.