In this study，the fulllength sequence of LbHXK gene was cloned from ‘Ningqi No. 1’ ( Lycium barbarum L.) fruit by RTPCR, and was 1 494 bp. LbHXK gene contained a complete open reading frame (ORF) and encoded a polypeptid of 498 amino acids. The protein molecular weight was 53.88 kD and the theoretical isoelectric point (pI) was 5.96. The LbHXK protein possessed a remarkable feature of the hexokinase family, including a ribosomal small subunit substructure of hexokinase (Gly91Val228) and a ribosomal big subunit substructure of hexokinase (Asn229Asp476). The tertiary structure of protein was V type and had a glucosebinding domain. Homologous gene comparison indicated that LbHXK had more high similarity with tobacco hexokinase genes (93.36% homology) in Solanaceae. The change of LbHXK expression was founded in different plant tissues by quantitative realtime PCR. LbHXK expressed in the highest in leaves and the lowest in roots, and there was no significant difference between different plant tissues. LbHXK expression of fruits showed a trend on first rising and then falling during fruit ripening, among which, reached on the highest level at coloring fruit phase (22 days after full bloom), and reached the lowest at the fruit ripening stage, and was significantly higher at earlymiddle stage than at latter stage of fruit development. This study would providd a foundation on further researching for function of LbHXK.