The dormancy associated MADSbox (DAM) gene plays an important role in the induction of plant bud dormancy. In this study, we used the different stages of Cerasus humilis ‘Nongda 4’ as materials to obtain the ChDAM gene using PCR technology. The gene has a total length of 705 bp and encodes 234 amino acids. Multisequence comparison and domain analysis found that the gene encoded protein has a typical MADSbox domain and Kbox domain, and its amino acid sequence is more homologous to peach DAM5 protein. The promoter analysis found that the expression of the ChDAM5 gene may be regulated by low temperature and abscisic acid. Realtime quantitative PCR results found that the expression of the ChDAM5 gene gradually increased with the process of dormancy induction. So it was speculated that the gene played a key role in inducing C. humilis dormancy, laying the foundation for further indepth metabolic pathway research.