Whirly family is a kind of plantspecific transcription factors, which play myriad roles both in the nucleus and organelles. Based on the whole genome data of Mangifera indica(mango), using bioinformatics methods to analyze the sequence of mango Whirly family genes, we studied the relative expression level of Whirly gene family by qRTPCR during the infection of Colletotrichum gloeosporioides(Cg) and Xanthomonas campestris pv. mangiferaeindicae(Xcm). The results showed that： (1) three Whirly family members, named MiWHY1, MiWHY2 and MiWHY3, were identified from the genome of mango. (2) The proteins of Whirly in mango were unstable hydrophilic basic proteins. The phylogenetic tree shows that the Whirly genes of mango are closely related to the Whirly genes of Solanum lycopersicum, Populus trichocarpa and Manihot esculenta, and there was a highly conserved sequence Motif1 in mango and Solanum lycopersicum, Populus trichocarpa and Manihot esculenta Whirly family. The Whirly family of mango contains the Whirly superfamily conservative domain, the main structural components of which are random coil and αhelix. The tetramer structure of conservative domain is similar to the tetramer structure of potato Whirly proteins. (3) qRTPCR results showed that the relative expression of mango Whirly genes were significantly upregulated during Cg infection. The relative expression levels of MiWHY1 and MiWHY3 were significantly upregulated at 12 h during Xcm infection. It was preliminarily determined that the mango Whirly gene expression responded to the infection of Cg and Xcm. In conclusion, there are three Whirly gene family members in mangosteen, and their expression level is stimulated during the infection process of pathogenic bacteria, which lays the foundation for the followup study of the functions and mechanisms of the mango Whirly gene family members.